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Text Box: (3) bean beetle in Trizol Text Box: Text Box: (3) Trizol reagent Text Box: Text Box: (1) bean beetles in 1.5 ml tubes Text Box: Text Box: (1) Mr. Madison transfers beetles Text Box: Text Box: (4) vortexing of crushed beetles Text Box: Text Box: (2) Mr. Harris- crushing of beetles Text Box: Text Box: (1) take bugs & transfer into 1.5 ml tube (2) crunch bugs with ethanol-wiped aluminum rod or plastic pestle (3) add 0.2 ml Trizol (4) mix by vortexing for 1 min (generates homogenate of bug & Trizol) (5) store at -20 Celsius until used (6) thaw at room temperate (7) vortex for 1 min (8) boil at 55 C for 3 hours